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Anti-PGAM1 Antibody (6H458) 是一种 Rabbit 抗体,靶向 PGAM1。Anti-PGAM1 Antibody (6H458) 可用于 WB,IHC,FCM。
Anti-PGAM1 Antibody (6H458) 是一种 Rabbit 抗体,靶向 PGAM1。Anti-PGAM1 Antibody (6H458) 可用于 WB,IHC,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,485 | 5日内发货 | |
100 μL | ¥ 2,480 | 5日内发货 |
产品描述 | Anti-PGAM1 Antibody (6H458) is a Rabbit antibody targeting PGAM1. Anti-PGAM1 Antibody (6H458) can be used in WB,IHC,FCM. |
别名 | Phosphoglycerate mutase isozyme B, Phosphoglycerate mutase 1, PGAM-B, PGAMA, PGAM 1, EC 5.4.2.4, EC 5.4.2.11, BPG-dependent PGAM 1 |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of PGAM1 on different lysates using anti-PGAM1 antibody at 1/2,000 dilution. Positive control: Lane 1: A431, Lane 2: A549, Lane 3: Rat brain, Lane 4: Mouse brain. 2. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PGAM1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PGAM1 antibody. Counter stained with hematoxylin. 4. Flow cytometric analysis of Jurkat cells with PGAM1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC: 1:50-100; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Members of the PGAM (phosphoglycerate mutase) family of proteins are important components of glucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism. They are responsible for catalyzing the transfer of phospho groups between the carbon atoms of phosphoglycerates. In mammals there are two types of PGAM isozymes: PGAM1 (also known as PGAMB) and PGAM2 (also known as PGAMA). In the cell, PGAM1 and PGAM2 exist as either homodimers or heterodimers and are responsible for the interconversion of 3-phosphoglycerate and 2-phosphoglycerate. PGAM2 homodimers are expressed in skeletal muscle, mature sperm cells and heart; PGAM1 homodimers are found in most other tissues; and PGAM1/PGAM2 heterodimers are found exclusively in the heart. PGAM4, also known as PGAM3, is a protein formerly considered to be specific to humans. Initially the PGAM4 gene was described as a pseudogene but it is now known to encode a functional protein at least 25 million years old. The gene encoding PGAM4 is believed to have originated by retrotransposition, with the original copy being the PGAM1 gene. |
偶联 | Unconjugated |
Uniprot ID |
分子量 | Theoretical: 29 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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