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Anti-PDK2 Antibody (1C819)

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Anti-PDK2 Antibody (1C819)
产品编号 TMAC-03140
别名 Pyruvate dehydrogenase lipoamide kinase isozyme 2, mitochondrial, Pyruvate dehydrogenase kinase, isozyme 2, Pyruvate dehydrogenase kinase isoform 2, PDK2_HUMAN, PDK 2, PDHK2, mitochondrial, [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2

Anti-PDK2 Antibody (1C819) 是一种 Rabbit 抗体，靶向 PDK2。Anti-PDK2 Antibody (1C819) 可用于 WB,IHC,FCM,IP。

Anti-PDK2 Antibody (1C819)

Anti-PDK2 Antibody (1C819)

Rating icon 还可以
Anti-PDK2 Antibody (1C819)
产品编号 TMAC-03140 别名 Pyruvate dehydrogenase lipoamide kinase isozyme 2, mitochondrial, Pyruvate dehydrogenase kinase, isozyme 2, Pyruvate dehydrogenase kinase isoform 2, PDK2_HUMAN, PDK 2, PDHK2, mitochondrial, [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2

Anti-PDK2 Antibody (1C819) 是一种 Rabbit 抗体，靶向 PDK2。Anti-PDK2 Antibody (1C819) 可用于 WB,IHC,FCM,IP。

规格价格库存数量
50 μL
¥ 1,485
5日内发货
100 μL
¥ 2,495
5日内发货
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产品介绍

生物活性
产品描述
Anti-PDK2 Antibody (1C819) is a Rabbit antibody targeting PDK2. Anti-PDK2 Antibody (1C819) can be used in WB,IHC,FCM,IP.
别名Pyruvate dehydrogenase lipoamide kinase isozyme 2, mitochondrial, Pyruvate dehydrogenase kinase, isozyme 2, Pyruvate dehydrogenase kinase isoform 2, PDK2_HUMAN, PDK 2, PDHK2, mitochondrial, [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of PDK2 on rat heart tissue lysate using anti-PDK2 antibody at 1/500 dilution.
2. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PDK2 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PDK2 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-PDK2 antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-PDK2 antibody. Counter stained with hematoxylin.
6. Flow cytometric analysis of SH-SY-5Y cells with PDK2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
应用
WB
IHC
FCM
IP
推荐剂量
WB: 1:500; IHC: 1:50-200; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Pyruvate dehydrogenase kinase family members (PDK1, 2, 3, 4) are serine kinases that catalyze the phosphorylation of the E1α subunit of the pyruvate dehydrogenase complex (PDC). PDC activity is controlled through phosphorylation and dephosphorylation of the E1α subunit, which leads to inactivation and reactivation, respectively. The core of PDC is composed of sixty dihydrolypoyl acetyltransferase (E2) subunits that bind directly to PDK2 and enhance PDK2 kinase activity. Upregulation of PDK isoenzymes occurs during starvation conditions, rerouting acetyl-CoA generation by facilitating fatty acid oxidation. PDKs contain five conserved regions and are mechanistically similar to bacterial His-kinases, in that both require Histidine residues for activity. In mammals, transcripts for PDK2 are ubiquitously expressed with high levels in heart and skeletal muscle and decreased levels in spleen and lung.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
Q15119
化学信息
分子量Theoretical: 46 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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