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Anti-NCAM1 Antibody (1L189)
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产品编号 TMAC-02752
别名 neural cell adhesion molecule 1, NCAM, MSK39, CD56
Anti-NCAM1 Antibody (1L189) 是一种 Rabbit 抗体，靶向 NCAM1。Anti-NCAM1 Antibody (1L189) 可用于 WB,ICC/IF,IHC,IP,FCM。
Anti-NCAM1 Antibody (1L189)
Anti-NCAM1 Antibody (1L189)
还可以产品编号 TMAC-02752 别名 neural cell adhesion molecule 1, NCAM, MSK39, CD56
Anti-NCAM1 Antibody (1L189) 是一种 Rabbit 抗体，靶向 NCAM1。Anti-NCAM1 Antibody (1L189) 可用于 WB,ICC/IF,IHC,IP,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,495 | 5日内发货 | |
| 100 μL | ¥ 2,490 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-NCAM1 Antibody (1L189) is a Rabbit antibody targeting NCAM1. Anti-NCAM1 Antibody (1L189) can be used in WB,ICC/IF,IHC,IP,FCM. |
| 别名 | neural cell adhesion molecule 1, NCAM, MSK39, CD56 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,zebrafish |
| 验证活性 | 1. Western blot analysis of NCAM on different lysates using anti-NCAM antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY-5Y, Lane 2: Human brain. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NCAM antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded zebrafish kidney tissue using anti-NCAM antibody. Counter stained with hematoxylin. 4. ICC staining NCAM in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining NCAM in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining NCAM in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of SH-SY-5Y cells with NCAM antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Neural cell adhesion molecules (NCAMs) are a family of closely related cell surface glycoproteins involved in cell to cell interactions during growth and thought to play an important role in embryogenesis and development. The expression of these molecules is widespread in all three germ layers during embryogenesis, but is more restrictive in adult tissues. NCAM expression is observed in a variety of human tumors including neuroblastomas, rhabdo-myosarcomas, Wilms' tumor, Ewing's sarcoma and some primitive myeloid malignancies. Multiple isoforms of NCAM have been reported in both mouse and human brain tissue. In humans, NCAMs arise from differential splicing and use of alternative polyadenylation sites of a single gene mapping to 11q23. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 100-150 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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