Anti-LEF1 Polyclonal Antibody is a Rabbit antibody targeting LEF1. Anti-LEF1 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Rabbit)

1. Sample: LympHnodes (Mouse) Lysate at 30 μg
Primary: Anti-LEF-1 (TMAB-01058) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
Predicted band size: 44 kDa
Observed band size: 47 kDa
2. Sample:
Lane 1: Mouse Thymus tissue lysates
Lane 2: Mouse Spleen tissue lysates
Primary: Anti-LEF-1 (TMAB-01058) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 55 kDa
3. Blank control (black line): Molt4. Primary Antibody (green line): Rabbit Anti-LEF-1 antibody (TMAB-01058)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Negative control (white blue line): PBS
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB: 1:500-2000; FCM: 1ug/test

Polyclonal

KLH conjugated synthetic peptide: human LEF-1

Human

LEF1

Lymphoid enhancer-binding factor 1

PTC & Wnt pathway,Mesoderm,HMG Box,Transcription Factors,Mesoderm,Nuclear

Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.