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Anti-Lactoylglutathione lyase/GLO1 Antibody (8P849)

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Anti-Lactoylglutathione lyase/GLO1 Antibody (8P849)
产品编号 TMAC-01668
别名 S-D-lactoylglutathione methylglyoxal lyase, Methylglyoxalase, Lactoylglutathione lyase, Ketone-aldehyde mutase, Glyoxalase I (Glx I), GLO1, Aldoketomutase

Anti-Lactoylglutathione lyase/GLO1 Antibody (8P849) 是一种 Rabbit 抗体,靶向 Lactoylglutathione lyase/GLO1。Anti-Lactoylglutathione lyase/GLO1 Antibody (8P849) 可用于 WB,ICC,IHC,FCM。

Anti-Lactoylglutathione lyase/GLO1 Antibody (8P849)

Anti-Lactoylglutathione lyase/GLO1 Antibody (8P849)

Rating icon 还可以
Anti-Lactoylglutathione lyase/GLO1 Antibody (8P849)
产品编号 TMAC-01668 别名 S-D-lactoylglutathione methylglyoxal lyase, Methylglyoxalase, Lactoylglutathione lyase, Ketone-aldehyde mutase, Glyoxalase I (Glx I), GLO1, Aldoketomutase

Anti-Lactoylglutathione lyase/GLO1 Antibody (8P849) 是一种 Rabbit 抗体,靶向 Lactoylglutathione lyase/GLO1。Anti-Lactoylglutathione lyase/GLO1 Antibody (8P849) 可用于 WB,ICC,IHC,FCM。

规格价格库存数量
50 μL
¥ 1,490
5日内发货
100 μL
¥ 2,495
5日内发货
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产品介绍

生物活性
产品描述
Anti-Lactoylglutathione lyase/GLO1 Antibody (8P849) is a Rabbit antibody targeting Lactoylglutathione lyase/GLO1. Anti-Lactoylglutathione lyase/GLO1 Antibody (8P849) can be used in WB,ICC,IHC,FCM.
别名S-D-lactoylglutathione methylglyoxal lyase, Methylglyoxalase, Lactoylglutathione lyase, Ketone-aldehyde mutase, Glyoxalase I (Glx I), GLO1, Aldoketomutase
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of GLO1 on different lysates using anti-GLO1 antibody at 1/500 dilution. Positive control: Lane 1: Mouse testis tissue, Lane 2: C2C12, Lane 3: 293, Lane 4: Rat spleen tissue.
2. Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-GLO1 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-GLO1 antibody. Counter stained with hematoxylin.
4. ICC staining GLO1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining GLO1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining GLO1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. Flow cytometric analysis of Hela cells with GLO1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
应用
推荐剂量
WB: 1:500-2000; IHC: 1:100-500; ICC: 1:100-500; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景The glyoxal pathway plays a role in the detoxification of glucose degradation products (GDP). Glyoxalase I (GLO1), a member of the glyoxalase family, is effective in eliminating GDP. Overexpression or silencing of Glyoxalase I in mice brain suggests an association between Glyoxalase I and anxiety. Glyoxalase I has three isoforms generated from two alleles in the genome which forms two homodimers and one heterodimer, each subunit binding one zinc ion. Research demonstrates that GLO1 gene expression is induced in colon carcinoma. Both an insulin response element (IRE), and a zinc metal response element (MRE) in the promoter region of the GLO1 gene have been identified.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
化学信息
分子量Theoretical: 21 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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