Anti-Integrin alpha 1 Polyclonal Antibody is a Rabbit antibody targeting Integrin alpha 1. Anti-Integrin alpha 1 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Rat (predicted:Mouse,Cow,Horse)

1. Blank control: RSC96 cells (blue). Primary Antibody: Rabbit Anti-Integrin alpha 1 antibody (TMAB-00978), Dilution: 5 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange),used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
2. Blank control: U937 (blue). Primary Antibody: Rabbit Anti-Integrin alpha 1 antibody (TMAB-00978), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (TMAB-00978, 1 μg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice.
3. Sample:
Lane 1: Hela (Human) Cell Lysate at 30 μg
Lane 2: 293T (Human) Cell Lysate at 30 μg
Lane 3: U87MG (Human) Cell Lysate at 30 μg
Lane 4: HepG2 (Human) Cell Lysate at 30 μg
Lane 5: A549 (Human) Cell Lysate at 30 μg
Primary: Anti-Integrin alpha 1 (TMAB-00978) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 150 kDa
Observed band size: 160 kDa

WB: 1:500-2000; FCM: 1μg /test

Polyclonal

KLH conjugated synthetic peptide: human Integrin alpha 1

Human

Basal Lamina,Alpha,Surface Molecules