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别名 RCCP2, MET proto-oncogene, receptor tyrosine kinase, HGFR, DFNB97, c-Met, AUTS9
Anti-HGFR/c-Met Antibody (4N790) 是一种 Rabbit 抗体,靶向 HGFR/c-Met。Anti-HGFR/c-Met Antibody (4N790) 可用于 FCM,ICC/IF,IHC,WB。
Anti-HGFR/c-Met Antibody (4N790) 是一种 Rabbit 抗体,靶向 HGFR/c-Met。Anti-HGFR/c-Met Antibody (4N790) 可用于 FCM,ICC/IF,IHC,WB。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,485 | 5日内发货 | |
| 100 μL | ¥ 2,485 | 5日内发货 |
| 产品描述 | Anti-HGFR/c-Met Antibody (4N790) is a Rabbit antibody targeting HGFR/c-Met. Anti-HGFR/c-Met Antibody (4N790) can be used in FCM,ICC/IF,IHC,WB. |
| 别名 | RCCP2, MET proto-oncogene, receptor tyrosine kinase, HGFR, DFNB97, c-Met, AUTS9 |
| Ig Type | IgG |
| 克隆号 | 4N790 |
| 交叉反应 | Human |
| 验证活性 | 1. Western blot analysis of Met on different lysates using anti-Met antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: HepG2. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Met antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Met antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Met antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Met antibody. Counter stained with hematoxylin. 6. ICC staining Met in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of Hela cells with Met antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
| 应用 | FCMICC/IFIHCWB |
| 推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | The c-Met oncogene was originally isolated from a chemical carcinogen-treated human osteogenic sarcoma cell line by transfection analysis in NIH/3T3 cells. The Met proto-oncogene product was identified as a transmembrane receptor-like protein with tyrosine kinase activity that is expressed in many tissues. A high proportion of spontaneous NIH/3T3 transformants overexpress c-Met and by transfection analysis the c-Met proto-oncogene has been shown to exhibit transforming activity. Tyrosine phosphorylation of apparently normal Met protein has also been observed in certain human gastric carcinoma cell lines . The c-Met gene product has been identified as the cell-surface receptor for hepatocyte growth factor, a plasminogen-like protein thought to be a humoral mediator of liver regeneration. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 155 kDa. |
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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