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顶部生物活性储存和溶解信息计算器

Anti-eIF4A3 Antibody (7D278)

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Anti-eIF4A3 Antibody (7D278)
产品编号 TMAC-01272
别名 NUK34, Nuclear matrix protein 265, NMP265, NMP 265, IF4A3_HUMAN, hNMP 265, Eukaryotic translation initiation factor 4A isoform 3, Eukaryotic initiation factor 4A-like NUK-34, Eukaryotic initiation factor 4A-III, eIF4A-III, eIF-4A-III, eIF4AIII, EIF4A3, DEAD box protein 48, DDX48, ATP-dependent RNA helicase eIF4A-3, ATP-dependent RNA helicase DDX48

Anti-eIF4A3 Antibody (7D278) 是一种 Rabbit 抗体，靶向 eIF4A3。Anti-eIF4A3 Antibody (7D278) 可用于 WB,ICC,IF,IHC,FCM,IP。

Anti-eIF4A3 Antibody (7D278)

Anti-eIF4A3 Antibody (7D278)

Rating icon 还可以
Anti-eIF4A3 Antibody (7D278)
产品编号 TMAC-01272 别名 NUK34, Nuclear matrix protein 265, NMP265, NMP 265, IF4A3_HUMAN, hNMP 265, Eukaryotic translation initiation factor 4A isoform 3, Eukaryotic initiation factor 4A-like NUK-34, Eukaryotic initiation factor 4A-III, eIF4A-III, eIF-4A-III, eIF4AIII, EIF4A3, DEAD box protein 48, DDX48, ATP-dependent RNA helicase eIF4A-3, ATP-dependent RNA helicase DDX48

Anti-eIF4A3 Antibody (7D278) 是一种 Rabbit 抗体，靶向 eIF4A3。Anti-eIF4A3 Antibody (7D278) 可用于 WB,ICC,IF,IHC,FCM,IP。

规格价格库存数量
50 μL
¥ 1,480
5日内发货
100 μL
¥ 2,495
5日内发货
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产品介绍

生物活性
产品描述
Anti-eIF4A3 Antibody (7D278) is a Rabbit antibody targeting eIF4A3. Anti-eIF4A3 Antibody (7D278) can be used in WB,ICC,IF,IHC,FCM,IP.
别名NUK34, Nuclear matrix protein 265, NMP265, NMP 265, IF4A3_HUMAN, hNMP 265, Eukaryotic translation initiation factor 4A isoform 3, Eukaryotic initiation factor 4A-like NUK-34, Eukaryotic initiation factor 4A-III, eIF4A-III, eIF-4A-III, eIF4AIII, EIF4A3, DEAD box protein 48, DDX48, ATP-dependent RNA helicase eIF4A-3, ATP-dependent RNA helicase DDX48
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of eIF4A3 on SiHa cell lysates using anti-eIF4A3 at 1/500 dilution.
2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-eIF4A3 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-eIF4A3 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-eIF4A3 antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-eIF4A3 antibody. Counter stained with hematoxylin.
6. ICC staining eIF4A3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining eIF4A3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. ICC staining eIF4A3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
9. Flow cytometric analysis of MCF-7 cells with eIF4A3 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
应用
WB
ICC
IF
IHC
FCM
IP
推荐剂量
WB: 1:500-1000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景ATP-dependent RNA helicase. Involved in pre-mRNA splicing as component of the spliceosome. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: human eIF4A3 aa 1-150
抗原种属
human
Uniprot ID
P38919
化学信息
分子量Theoretical: 47 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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