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Anti-CYC1 Antibody (5W832)
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产品编号 TMAH-00325
别名 Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit, mitochondrial, heme protein, EC:7.1.1.8, Cytochrome c-1, Cytochrome c1, Cytochrome b-c1 complex subunit 4, Complex III subunit IV, Complex III subunit 4
Anti-CYC1 Antibody (5W832) 是一种 Mouse 抗体，靶向 CYC1。Anti-CYC1 Antibody (5W832) 可用于 ELISA, WB, FCM。
Anti-CYC1 Antibody (5W832)
Anti-CYC1 Antibody (5W832)
还可以产品编号 TMAH-00325 别名 Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit, mitochondrial, heme protein, EC:7.1.1.8, Cytochrome c-1, Cytochrome c1, Cytochrome b-c1 complex subunit 4, Complex III subunit IV, Complex III subunit 4
Anti-CYC1 Antibody (5W832) 是一种 Mouse 抗体，靶向 CYC1。Anti-CYC1 Antibody (5W832) 可用于 ELISA, WB, FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,305 | 5日内发货 | |
| 100 μL | ¥ 2,190 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-CYC1 Antibody (5W832) is a Mouse antibody targeting CYC1. Anti-CYC1 Antibody (5W832) can be used in ELISA, WB, FCM. |
| 别名 | Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit, mitochondrial, heme protein, EC:7.1.1.8, Cytochrome c-1, Cytochrome c1, Cytochrome b-c1 complex subunit 4, Complex III subunit IV, Complex III subunit 4 |
| Ig Type | IgG1 |
| 克隆号 | 5W832 |
| 交叉反应 | Human, Mouse |
| 验证活性 | 1. Western Blot -Positive WB detected in: CYC1 antibody at 1:1000 -Lane 1: Hela whole cell lysate -Lane 2: HepG2 whole cell lysate -Lane 3: MCF-7 whole cell lysate -Lane 4: K562 whole cell lysate -Lane 5: U251 whole cell lysate -Lane 6: A549 whole cell lysate -Lane 7: NIH/3T3 whole cell lysate -Lane 8: PC-3 whole cell lysate -Secondary: Goat polyclonal to Mouse IgG at 1/20000 dilution -Predicted band size: 35 KDa -Observed band size: 35 KDa -Exposure time: 5min 2. Overlay Peak curve showing HepG2 cells stained with TMAH-00325 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| 应用 | |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 亚细胞定位 | Mitochondrion inner membrane; Single-pass membrane protein. |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein G purified |
| 性状 | Liquid |
| 缓冲液 | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
| 纯度 | >95% |
| 研究背景 | Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. Cytochrome c1 is a catalytic core subunit containing a c-type heme. It transfers electrons from the [2Fe-2S] iron-sulfur cluster of the Rieske protein to cytochrome c. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein: Human Cytochrome c1 Protein (85-325AA) |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Tags & Cell Markers |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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