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Anti-CD40 Ligand Antibody (1A416)

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Anti-CD40 Ligand Antibody (1A416)
产品编号 TMAC-00711
别名 TRAP, TNFSF5IMD3, TNFSF5, T-BAM, IMD3, IGM, HIGM1, gp39, CD40LIGM, CD40LG, CD40L, CD40 Ligand, CD154

Anti-CD40 Ligand Antibody (1A416) 是一种 Rabbit 抗体，靶向 CD40 Ligand。Anti-CD40 Ligand Antibody (1A416) 可用于 WB,ICC/IF,IHC,FCM。

Anti-CD40 Ligand Antibody (1A416)

Anti-CD40 Ligand Antibody (1A416)

Rating icon 还可以
Anti-CD40 Ligand Antibody (1A416)
产品编号 TMAC-00711 别名 TRAP, TNFSF5IMD3, TNFSF5, T-BAM, IMD3, IGM, HIGM1, gp39, CD40LIGM, CD40LG, CD40L, CD40 Ligand, CD154

Anti-CD40 Ligand Antibody (1A416) 是一种 Rabbit 抗体，靶向 CD40 Ligand。Anti-CD40 Ligand Antibody (1A416) 可用于 WB,ICC/IF,IHC,FCM。

规格价格库存数量
50 μL
¥ 1,490
5日内发货
100 μL
¥ 2,495
5日内发货
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产品介绍

生物活性
产品描述
Anti-CD40 Ligand Antibody (1A416) is a Rabbit antibody targeting CD40 Ligand. Anti-CD40 Ligand Antibody (1A416) can be used in WB,ICC/IF,IHC,FCM.
别名TRAP, TNFSF5IMD3, TNFSF5, T-BAM, IMD3, IGM, HIGM1, gp39, CD40LIGM, CD40LG, CD40L, CD40 Ligand, CD154
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of CD40L on different cells lysates using anti-CD40L antibody at 1/500 dilution. Positive control: Lane 1: Hela, Lane 2: Mouse liver.
2. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti- CD40L antibody. Counter stained with hematoxylin.
3. ICC staining CD40L in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining CD40L in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining CD40L in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. Flow cytometric analysis of THP-1 cells with CD40L antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
应用
WB
ICC/IF
IHC
FCM
推荐剂量
WB: 1:500-1000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Resting B cells can be activated and clonally expanded into antibody-producing cells in response to a combination of cell contact and soluble signals provided by primed helper T (Th) cells. While cytokines IL-4 and IL-13 alone are inadequate for B cell activation, contact with Th cells seems to be sufficient for delivery of proliferative signals. CD40 and CD154 (also designated CD40L or TRAP) comprise a receptor ligand pair central to the transmission of this signal. CD40 is expressed on the surface of B cells and CD154 is expressed on activated T cells. In the presence of such stimulus, IL-4 and IL-13 are capable of triggering immunoglobulin class switching and secretion of IgE. CD154 is a 261 amino acid protein that is is expressed as a soluble cytokine as well as a homotrimeric type II transmembrane protein. Its expression is tightly regulated, and abnormal levels of CD154 are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Mutations in the gene encoding for CD154 are implicated in hyper-IgM immunodeficiency syndrome type 1.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
P29965
化学信息
分子量Theoretical: 36 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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关键词

TNFSF-5TNFSF 5IMD-3IMD 3HIGM-1HIGM 1CD-154CD 154

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