Anti-3-Nitro-L-tyrosine Polyclonal Antibody is a Rabbit antibody targeting 3-Nitro-L-tyrosine. Anti-3-Nitro-L-tyrosine Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Species independent

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Nitro tyrosine Polyclonal Antibody, Unconjugated (TMAB-00003) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Blank control: Hela (blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
Isotype Control Antibody: Rabbit Igg (orange);
Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue),
Dilution: 1:100 in 1 X PBS containing 0.5% BSA;
Primary Antibody Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA (green).
3. Sample: 3-Nitrotyrosine-BSA conjugate Protein
Primary: Anti-3-Nitrotyrosine (TMAB-00003) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:50-200; FCM: 1μg/Test

Polyclonal

KLH conjugated Nitrotyrosine

Signal transduction proteins ELISA kits,Amino Acids