Human respiratory syncytial virus RSV TMPY-06368

Human respiratory syncytial virus (RSV) (A, strain Long) Fusion glycoprotein F0/RSV-F Protein (His)

产品编号 TMPY-06368 Human respiratory syncytial virus (RSV) (A, strain Long) Fusion glycoprotein F0/RSV-F Protein (His)

Human respiratory syncytial virus (RSV) (A, strain Long) Fusion glycoprotein F0/RSV-F Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 56.92 kDa and the accession number is P12568.

规格	价格	库存	数量
100 μg	¥ 6,390	5日内发货

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常见问题解答

问化合物如何用于动物实验？如何确立剂量、给药方式等？

答同一个化合物对不同动物模型、不同疾病模型的用法不一样，建议根据实验目的，查询文献选择用法。常见的给药方式包括腹腔注射、灌胃等。由于首过效应，一般可以灌胃的也可以腹腔注射，可以腹腔注射的不一定适合灌胃。不同种属的给药剂量可以根据官网的表格换算。但仅供参考，实际应用中可能未必合适或者准确，尽量以具体某种动物给药剂量的参考文献为主。我们的化合物都可以进行细胞动物实验，但有些化合物还没有用于动物实验的文献，这种情况下，您可以尝试进行动物实验，但我们无法提供使用方式，也无法保证药效。

问细胞实验中，抑制剂如何溶解？

答用 DMSO 配置较高浓度母液，母液存于 -80℃，建议多分装几管，一周内使用的存于 4℃，避免反复冻融。实验时用 pbs、生理盐水、培养基、水这些稀释，细胞实验DMSO的终浓度不超过 0.1%。如果超过 0.1%，则需要在做预试验的时候，先进行溶剂阴性对照实验，排除溶剂对细胞的影响。

问超声应使用什么仪器？

答建议使用“超声清洗仪”，不推荐用“超声破碎仪”。

问抑制剂能否用于动物实验？

答可以的，我们的抑制剂都可以用于动物/体内实验。但有些化合物还没有用于动物实验的文献，这种情况下，建议您先做预实验，确保研究的可行性。

问产品溶解需要超声吗？如果没有超声仪怎么办？

答超声可以加快粉末的溶解速度，如果您的化合物溶解不了，建议您尝试超声。如果您没有超声仪，建议您选择较低母液浓度，或者较少粉末量，尝试溶解。

产品信息

生物活性	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述	Human respiratory syncytial virus (RSV) (A, strain Long) Fusion glycoprotein F0/RSV-F Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 56.92 kDa and the accession number is P12568.
种属	RSV
表达系统	Baculovirus Insect Cells
标签	C-His
蛋白编号	P12568
别名	HRSVgp8,F Protein
蛋白构建	A DNA sequence encoding the Human respiratory syncytial virus (RSV) (A, strain Long) Fusion glycoprotein F0 (P12568) (Met1-Asn524) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Gln 26
蛋白纯度	> 95 % as determined by SDS-PAGE.
分子量	56.92 kDa (predicted); 75.98 and 61.80 kDa (reducing condition, due to glycosylation)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from 20 mM Tris, 300 mM NaCl, 10% glycerol, pH7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. It is classified within the genus pneumovirus of the family paramyxoviridae. Like other members of the family, HRSV has two major surface glycoproteins (G and F) that play important roles in the initial stages of the infectious cycle. The G protein mediates attachment of the virus to cell surface receptors, while the F protein promotes fusion of the viral and cellular membranes, allowing entry of the virus ribonucleoprotein into the cell cytoplasm. The fusion (F) protein of RSV is synthesized as a nonfusogenic precursor protein (F), which during its migration to the cell surface is activated by cleavage into the disulfide-linked F1 and F2 subunits. This fusion is pH independent and occurs directly at the outer cell membrane, and the F2 subunit was identifed as the major determinant of RSV host cell specificity. The trimer of F1-F2 interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and induces the fusion between host cell and virion membranes. Notably, RSV fusion protein is unique in that it is able to interact directly with heparan sulfate and therefore is sufficient for virus infection. Furthermore, the fusion protein is also able to trigger p53-dependent apoptosis.