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Anti-PPAR alpha/PPARA Antibody (3P706)
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产品编号 TMAB-01563
Anti-PPAR alpha/PPARA Antibody (3P706) 是一种 Mouse 抗体，靶向 PPAR alpha/PPARA。Anti-PPAR alpha/PPARA Antibody (3P706) 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。
Anti-PPAR alpha/PPARA Antibody (3P706)
Anti-PPAR alpha/PPARA Antibody (3P706)
还可以产品编号 TMAB-01563
Anti-PPAR alpha/PPARA Antibody (3P706) 是一种 Mouse 抗体，靶向 PPAR alpha/PPARA。Anti-PPAR alpha/PPARA Antibody (3P706) 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,570 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
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产品介绍
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抗原信息
| 产品描述 | Anti-PPAR alpha/PPARA Antibody (3P706) is a Mouse antibody targeting PPAR alpha/PPARA. Anti-PPAR alpha/PPARA Antibody (3P706) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Ig Type | IgG1 |
| 克隆号 | 3P706 |
| 交叉反应 | Human,Mouse |
| 验证活性 | 1. Sample: Lane 1: Skeletal muscle (Mouse) Lysate at 40 μg Lane 2: Liver (Mouse) Lysate at 40 μg Lane 3: Heart (Mouse) Lysate at 40 μg Lane 4: Kidney (Mouse) Lysate at 40 μg Primary: Anti-PPAR alpha (TMAB-01563) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 51 kDa Observed band size: 51 kDa 2. Sample: Lane 1: Hela (Human) Cell Lysate at 30 μg Lane 2: Jurkat (Human) Cell Lysate at 30 μg Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 μg Primary: Anti-PPAR alpha (TMAB-01563) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:400-800; ICC/IF: 1:20-100; IF: 1:20-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 亚细胞定位 | Nucleus. |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein G purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | Peroxisome proliferators are nongenotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed Peroxisome Proliferator Activated Receptors (PPARs). Nuclear hormone receptors are ligand dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR alpha, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex. |
| 免疫原 | KLH conjugated synthetic peptide: human PPAR alpha |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Metabolism of lipids and lipoproteins,Response to hypoxia,Nuclear hormone receptors,Metabolism,Zinc Finger,Fatty acids,Lipid metabolism,Mitochondrial transcription,Hypoxia,Mitochondrial Biogenesis,Fatty acid oxidation,Obesity |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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