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Anti-Phospho-PRKCA (Thr198) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-PRKCA (Thr198)。Anti-Phospho-PRKCA (Thr198) Polyclonal Antibody 可用于 WB,IP,IF。
Anti-Phospho-PRKCA (Thr198) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-PRKCA (Thr198)。Anti-Phospho-PRKCA (Thr198) Polyclonal Antibody 可用于 WB,IP,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,485 | 5日内发货 | |
100 μL | ¥ 2,490 | 5日内发货 |
产品描述 | Anti-Phospho-PRKCA (Thr198) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PRKCA (Thr198). Anti-Phospho-PRKCA (Thr198) Polyclonal Antibody can be used in WB,IP,IF. |
别名 | Protein kinase C zeta, Protein kinase C gamma, Protein kinase C epsilon, Protein kinase C delta, Protein kinase C beta, Protein kinase C alpha, Protein kinase C, PRKCZ, PRKCG, PRKCE, PRKCD, PRKCB2, PRKCB1, PRKCB, PRKCA (p-Thr198), PRKCA (p-T198), PRKCA, p-PRKCA (Thr198), p-PRKCA (T198), PKCG, PKCE, PKCD, PKCB, PKC-alpha, PKC-A, PKCA, PKC2, PKC zeta, PKC gamma, PKC epsilon, PKC delta, PKC beta, PKC alpha, Phospho-PRKCA (T198), KPCA_HUMAN |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of PKAα cat phosphorylation in non-transfected (A) and human PKAα cat transfected (B) 293T whole cell lysates. 2. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization. |
应用 | |
推荐剂量 | WB: 1:100-1000; IP: 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
构建方式 | Polyclonal Antibody |
纯化方式 | Immunogen affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similarity), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on Serine 133, which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the Pol II transcription initiation complex. The phosphorylation at Threonine 198 is cAMP dependent. |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | A short amino acid sequence containing Thr 198 phosphorylated PKAα cat of human origin |
抗原种属 | human |
分子量 | Theoretical: PKAα cat: 40, PKAβ cat: 53, PKAγ cat: 39-40 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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