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Anti-NOX4 Antibody (4J332)
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产品编号 TMAC-02745
别名 RENOX, Renal NAD(P)H-oxidase, NOX4, NADPH oxidase 4, Kidney superoxide-producing NADPH oxidase, Kidney oxidase-1 (KOX-1)
Anti-NOX4 Antibody (4J332) 是一种 Rabbit 抗体，靶向 NOX4。Anti-NOX4 Antibody (4J332) 可用于 WB,ICC,IHC,IP。
Anti-NOX4 Antibody (4J332)
Anti-NOX4 Antibody (4J332)
还可以产品编号 TMAC-02745 别名 RENOX, Renal NAD(P)H-oxidase, NOX4, NADPH oxidase 4, Kidney superoxide-producing NADPH oxidase, Kidney oxidase-1 (KOX-1)
Anti-NOX4 Antibody (4J332) 是一种 Rabbit 抗体，靶向 NOX4。Anti-NOX4 Antibody (4J332) 可用于 WB,ICC,IHC,IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,485 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-NOX4 Antibody (4J332) is a Rabbit antibody targeting NOX4. Anti-NOX4 Antibody (4J332) can be used in WB,ICC,IHC,IP. |
| 别名 | RENOX, Renal NAD(P)H-oxidase, NOX4, NADPH oxidase 4, Kidney superoxide-producing NADPH oxidase, Kidney oxidase-1 (KOX-1) |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of NOX4 on PC-12 cell lysates using anti-NOX4 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti-NOX4 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NOX4 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-NOX4 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-NOX4 antibody. Counter stained with hematoxylin. 6. ICC staining NOX4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining NOX4 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining NOX4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| 应用 | |
| 推荐剂量 | WB: 1:1000; IHC: 1:50-200; ICC: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | The superoxide-generating NADPH oxidase includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with the flavocytochrome, cytochrome b558, to form the active enzyme complex. The p22 and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. Nox4 (also known as Renox) is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. Nox4 is also expressed in fetal tissues, placenta, glioblastoma and vascular cells. Like gp91-phox, the enzymatic activity of Nox4 produces superoxide anions. In vascular cells, the addition of Angiotensin II increases Nox4 expression, which suggests a role for Nox4 in vascular oxidative stress response. The gene encoding human Nox4 maps to chromosome 11q14.2-q21. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 67 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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