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Anti-MPO Antibody (4N671)

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Anti-MPO Antibody (4N671)
产品编号 TMAC-02717
别名 myeloperoxidase

Anti-MPO Antibody (4N671) 是一种 Rabbit 抗体，靶向 MPO。Anti-MPO Antibody (4N671) 可用于 WB,ICC/IF,IHC。

Anti-MPO Antibody (4N671)

Anti-MPO Antibody (4N671)

Rating icon 还可以
Anti-MPO Antibody (4N671)
产品编号 TMAC-02717 别名 myeloperoxidase

Anti-MPO Antibody (4N671) 是一种 Rabbit 抗体，靶向 MPO。Anti-MPO Antibody (4N671) 可用于 WB,ICC/IF,IHC。

规格价格库存数量
50 μL
¥ 1,495
5日内发货
100 μL
¥ 2,485
5日内发货
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产品介绍

生物活性
产品描述
Anti-MPO Antibody (4N671) is a Rabbit antibody targeting MPO. Anti-MPO Antibody (4N671) can be used in WB,ICC/IF,IHC.
别名myeloperoxidase
Ig Type
IgG
交叉反应
Human
验证活性
1. Western blot analysis of Myeloperoxidase on HL-60 cell lysates using anti-Myeloperoxidase antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Myeloperoxidase antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Myeloperoxidase antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Myeloperoxidase antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Myeloperoxidase antibody. Counter stained with hematoxylin.
6. ICC staining Myeloperoxidase in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining Myeloperoxidase in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. ICC staining Myeloperoxidase in AGS cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
应用
WB
ICC/IF
IHC
推荐剂量
WB: 1:500-1000; IHC: 1:50-200; ICC/IF: 1:50-200
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景The heme protein myeloperoxidase (MPO) is a major component of azurophilic granules of neutrophils and polymorphonuclear leukocytes. Optimal oxygen-dependent microbiocidal activity depends on MPO as the critical enzyme for the generation of hypochlorous acid and other toxic oxygen products. The MPO precursor is synthesized during the promyelocytic stage of myeloid differentiation and is subsequently processed and transported intracellularly to the lysosomes. The precursor undergoes cotranslational N-linked glycosylation to produce a glycoprotein. Glucosidases in the endoplasmic reticulum (ER) or early cis Golgi convert the pro-MPO to a form which is sorted into a prelysosomal compartment, which undergoes final proteolytic maturation to native MPO, a pair of heavy-light protomers. In normal neutrophils, MPO is expressed as a dimer. Calreticulin, a calcium-binding protein residing in the ER, interacts specifically with fully glycosylated apopro-MPO. iMPO mRNA is abundant in human promyelocytic HL-60 and mouse myeloid leukemia NFS-60 cells. MPO is expressed at high levels in circulating neutrophils and monocytes but is not detectable in microglia, brain-specific macrophages or normal brain tissue.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
P05164
化学信息
分子量Theoretical: 84 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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