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Anti-MAPK1 Antibody (9E797)

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Anti-MAPK1 Antibody (9E797)
产品编号 TMAH-00727
别名 PRKM2, PRKM1, p42-MAPK, Mitogen-activated protein kinase 1, MAPK1, MAPK 2, MAPK 1, MAP kinase 2, MAP kinase 1, ERT1, ERK-2, ERK2

Anti-MAPK1 Antibody (9E797) 是一种抗体，靶向 MAPK1。Anti-MAPK1 Antibody (9E797) 可用于 ELISA, WB, IHC, IF。

Anti-MAPK1 Antibody (9E797)

Anti-MAPK1 Antibody (9E797)

Rating icon 还可以
Anti-MAPK1 Antibody (9E797)
产品编号 TMAH-00727 别名 PRKM2, PRKM1, p42-MAPK, Mitogen-activated protein kinase 1, MAPK1, MAPK 2, MAPK 1, MAP kinase 2, MAP kinase 1, ERT1, ERK-2, ERK2

Anti-MAPK1 Antibody (9E797) 是一种抗体，靶向 MAPK1。Anti-MAPK1 Antibody (9E797) 可用于 ELISA, WB, IHC, IF。

规格价格库存数量
50 μL
¥ 1,305
5日内发货
100 μL
¥ 2,190
5日内发货
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产品介绍

生物活性
产品描述
Anti-MAPK1 Antibody (9E797) is an antibody targeting MAPK1. Anti-MAPK1 Antibody (9E797) can be used in ELISA, WB, IHC, IF.
别名PRKM2, PRKM1, p42-MAPK, Mitogen-activated protein kinase 1, MAPK1, MAPK 2, MAPK 1, MAP kinase 2, MAP kinase 1, ERT1, ERK-2, ERK2
Ig Type
Rabbit IgG
克隆号
9E797
交叉反应
Human, Mouse, Rat
验证活性
1. Western Blot
-Positive WB detected in: Jurkat whole cell lysate, Hela whole cell lysate, HEK293 whole cell lysate, HepG2 whole cell lysate, A375 whole cell lysate, A549 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate
-All lanes: ERK2 antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 42, 37 kDa
-Observed band size: 42 kDa
2. IHC image of TMAH-00727 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
3. Immunofluorescence staining of Hela Cells with TMAH-00727 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
应用
ELISA
WB
IHC
IF
推荐剂量
WB:1:500-1:5000; IHC:1:50-1:200; IF:1:20-1:200.
抗体种类
Monoclonal
亚细胞定位Cytoplasm, cytoskeleton, spindle. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm. Membrane, caveola. Cell junction, focal adhesion.
构建方式Recombinant Antibody
纯化方式Affinity-chromatography
性状Liquid
缓冲液Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in response to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation. Phosphorylates CDK2AP2. Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
A synthetic peptide: Human ERK2
抗原种属
Human
基因ID
5594
Uniprot ID
P28482
研究领域
Neuroscience, Signal transduction, Stem cells
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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