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Anti-HSPB8 Antibody (1K143) 是一种 Rabbit 抗体,靶向 HSPB8。Anti-HSPB8 Antibody (1K143) 可用于 WB,ICC/IF,IHC。
Anti-HSPB8 Antibody (1K143) 是一种 Rabbit 抗体,靶向 HSPB8。Anti-HSPB8 Antibody (1K143) 可用于 WB,ICC/IF,IHC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,495 | 5日内发货 | |
100 μL | ¥ 2,490 | 5日内发货 |
产品描述 | Anti-HSPB8 Antibody (1K143) is a Rabbit antibody targeting HSPB8. Anti-HSPB8 Antibody (1K143) can be used in WB,ICC/IF,IHC. |
别名 | α-crystallin C chain, Small stress protein-like protein HSP22, Protein kinase H11, HspB8, HSP22, Heat shock protein β-8, Heat shock protein beta-8, E2-induced gene 1 protein, E2IG1, CRYAC, Alpha-crystallin C chain |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of Hsp22 on different lysates using anti-Hsp22 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2, Lane 2: Mouse skeletal muscle. 2. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Hsp22 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-Hsp22 antibody. Counter stained with hematoxylin. 4. ICC staining Hsp22 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Hsp22 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Hsp22 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
应用 | |
推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:100-500 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Crystallins are the major proteins expressed in the vertebrate eye lens, where they maintain the transparency and refractive index of the lens. Crystallins are divided into a, b and g families; b and g-crystallins compose a superfamily. Crystallins usually contain seven distinctive protein regions, including four homologous motifs, a connecting peptide, and N- and C-terminal extensions. a-crystallins consist of three gene products, aA, aB and aC-crystallin, which are members of the small heat shock protein family (HSP20). They are induced by heat shock, and act as molecular chaperones by holding denatured proteins in large soluble aggregates. However, unlike other molecular chaperones, a-crystallins do not renature these proteins. Research indicates that binding occurs between membranes and aC-crystallin. The binding site appears to be at the polar-apolar interface in membrane protein (MIP26) and aC-crystallin; the lipid bilayer becomes less mobile with aC-crystallin binding. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 22 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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