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Anti-Beta-Catenin Antibody (5O621)
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产品编号 TMAB-00229
Anti-Beta-Catenin Antibody (5O621) 是一种 Mouse 抗体，靶向 Beta-Catenin。Anti-Beta-Catenin Antibody (5O621) 可用于 WB。
Anti-Beta-Catenin Antibody (5O621)
Anti-Beta-Catenin Antibody (5O621)
还可以产品编号 TMAB-00229
Anti-Beta-Catenin Antibody (5O621) 是一种 Mouse 抗体，靶向 Beta-Catenin。Anti-Beta-Catenin Antibody (5O621) 可用于 WB。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,165 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 | |
| 200 μL | ¥ 2,795 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-Beta-Catenin Antibody (5O621) is a Mouse antibody targeting Beta-Catenin. Anti-Beta-Catenin Antibody (5O621) can be used in WB. |
| Ig Type | IgG |
| 克隆号 | 5O621 |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Sample: U87Mg (Human) Cell Lysate at 30 μg U2os (Human) Cell Lysate at 30 μg Primary: Anti-Beta catenin (TMAB-00229) at 1/5000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 92 kDa Observed band size: 92 kDa 2. Sample: Hela (Human) Cell Lysate at 30 μg NIH/3T3 (Mouse) Cell Lysate at 30 μg Medulld (Mouse) Lysate at 40 μg Testis (Mouse) Lysate at 40 μg Cerebrum (Rat) Lysate at 40 μg Primary: Anti-Beta catenin (TMAB-00229) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 92 kDa Observed band size: 95 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:1000-5000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 亚细胞定位 | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell junction. Cell membrane. Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole. Note=Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. The majority of beta-catenin is localized to the cell membrane. In interphase, colocalizes with CROCC between CEP250 puncta at the proximal end of centrioles, and this localization is dependent on CROCC and CEP250. In mitosis, when NEK2 activity increases, it localizes to centrosomes at spindle poles independent of CROCC. Co-localizes with CDK5 in the cell-cell contacts and plasma membrane of undifferentiated and differentiated neuroblastoma cells. |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein G purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Oct 2009]. |
| 免疫原 | Recombinant Protein: human Beta catenin |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Cytoplasmic,Catenins,Cadherins,beta catenin,Neurogenesis,ChIP antibodies,Leukocyte recruitment,Cadherins |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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