Anti-BAX Antibody (7Y415)

Name: Anti-BAX Antibody (7Y415)
Brand: TargetMol
SKU: TMAB-00185
Rating: 4.5 (1 reviews)

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产品编号 TMAB-00185

Anti-BAX Antibody (7Y415) 是一种 Mouse 抗体，靶向 BAX。Anti-BAX Antibody (7Y415) 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-BAX Antibody (7Y415)

还可以

产品编号 TMAB-00185

Anti-BAX Antibody (7Y415) 是一种 Mouse 抗体，靶向 BAX。Anti-BAX Antibody (7Y415) 可用于 WB,IHC-P,IHC-Fr,IF。

规格	价格	库存
50 μL	¥ 1,165	5日内发货
100 μL	¥ 1,970	5日内发货
200 μL	¥ 2,790	5日内发货

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生物活性

产品描述	Anti-BAX Antibody (7Y415) is a Mouse antibody targeting BAX. Anti-BAX Antibody (7Y415) can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type	IgG1
克隆号	7Y415
交叉反应	Human,Mouse (predicted:Rat)
验证活性	1. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Bax) Monoclonal Antibody, Unconjugated (TMAB-00185) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody for 90 minutes, and DAPI for nucleus staining. 2. Sample: Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-Bax (TMAB-00185) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 21 kDa Observed band size: 21 kDa 3. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Bax) Monoclonal Antibody, Unconjugated (TMAB-00185) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
应用	WB IHC-P IHC-Fr IF
推荐剂量	WB: 1:1000-1500; IHC-P: 1:100-500; IHC-Fr: 1:200-500; IF: 1:200-500
抗体种类	Monoclonal
宿主来源	Mouse
亚细胞定位	Isoform Alpha: Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Note=Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane. Isoform Beta: Cytoplasm. Isoform Gamma: Cytoplasm. Isoform Delta: Cytoplasm (Potential).
构建方式	Hybridoma Monoclonal Antibody
纯化方式	Protein G purified
性状	Liquid
缓冲液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景	The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008].

抗原信息

免疫原	KLH conjugated synthetic peptide: human Bax
抗原种属	Human
基因ID	581
Uniprot ID	Q07812
研究领域	Bcl 2 family,Metabolism,Bcl 2 family,Associated Proteins,Bcl2 Family,Apoptosis marker and proteins ELISA kits,Apoptosis