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Anti-ATP5B Antibody (8V910)
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产品编号 TMAC-00341
Anti-ATP5B Antibody (8V910) 是一种 Rabbit 抗体，靶向 ATP5B。Anti-ATP5B Antibody (8V910) 可用于 WB,IP,ICC/IF,IHC。
Anti-ATP5B Antibody (8V910)
Anti-ATP5B Antibody (8V910)
还可以产品编号 TMAC-00341
Anti-ATP5B Antibody (8V910) 是一种 Rabbit 抗体，靶向 ATP5B。Anti-ATP5B Antibody (8V910) 可用于 WB,IP,ICC/IF,IHC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,485 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
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| 产品描述 | Anti-ATP5B Antibody (8V910) is a Rabbit antibody targeting ATP5B. Anti-ATP5B Antibody (8V910) can be used in WB,IP,ICC/IF,IHC. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat,zebrafish |
| 验证活性 | 1. Western blot analysis of ATPB on different cells lysates using anti-ATPB antibody at 1/500 dilution. Positive control: Line1: Hela, Line2: HepG2, Line3: 293T. 2. Western blot analysis of ATPB on Zebrafish cells lysates using anti-ATPB antibody at 1/500 dilution. 3. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ATPB antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ATPB antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ATPB antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded huaman uterus tissue using anti-ATPB antibody. Counter stained with hematoxylin. 7. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ATPB antibody. Counter stained with hematoxylin. 8. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ATPB antibody. Counter stained with hematoxylin. 9. ICC staining ATPB in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. ICC staining ATPB in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 11. ICC staining ATPB in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:100-500; IP: 1:10-50 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 53 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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