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顶部生物活性储存和溶解信息计算器

Anti-ATF-5 Antibody (5U516)

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Anti-ATF-5 Antibody (5U516)
产品编号 TMAH-00086

Anti-ATF-5 Antibody (5U516) 是一种抗体，靶向 ATF-5。Anti-ATF-5 Antibody (5U516) 可用于 ELISA, WB, IHC。

Anti-ATF-5 Antibody (5U516)

Anti-ATF-5 Antibody (5U516)

Rating icon 还可以
Anti-ATF-5 Antibody (5U516)
产品编号 TMAH-00086

Anti-ATF-5 Antibody (5U516) 是一种抗体，靶向 ATF-5。Anti-ATF-5 Antibody (5U516) 可用于 ELISA, WB, IHC。

规格价格库存数量
50 μL
¥ 1,310
5日内发货
100 μL
¥ 2,195
5日内发货
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产品介绍

生物活性
产品描述
Anti-ATF-5 Antibody (5U516) is an antibody targeting ATF-5. Anti-ATF-5 Antibody (5U516) can be used in ELISA, WB, IHC.
Ig Type
Rabbit IgG
克隆号
5U516
交叉反应
Human, Rat
验证活性
1. Western Blot
-Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, THP-1 whole cell lysate, PC-3 whole cell lysate, Rat brain tissue
-All lanes: ATF5 antibody at 1:2000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 31 kDa
-Observed band size: 35 kDa
2. IHC image of TMAH-00086 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
应用
ELISA
WB
IHC
推荐剂量
WB:1:500-1:5000; IHC:1:50-1:200.
抗体种类
Monoclonal
亚细胞定位Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
构建方式Recombinant Antibody
纯化方式Affinity-chromatography
性状Liquid
缓冲液Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景Transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. Important regulator of the cerebral cortex formation, functions in cerebral cortical neuroprogenitor cells to maintain proliferation and to block differentiation into neurons. Must be down-regulated in order for such cells to exit the cycle and differentiate. Participates in the pathways by which SHH promotes cerebellar granule neuron progenitor cells proliferation. Critical for survival of mature olfactory sensory neurons (OSN), directs expression of OSN-specific genes. May be involved in osteogenic differentiation. Promotes cell proliferation and survival by inducing the expression of EGR1 sinergistically with ELK1. Once acetylated by EP300, binds to ARE sequences on target genes promoters, such as BCL2 and EGR1. Plays an anti-apoptotic role through the transcriptional regulation of BCL2, this function seems to be cell type-dependent. Cooperates with NR1I3/CAR in the transcriptional activation of CYP2B6 in liver. In hepatic cells, represses CRE-dependent transcription and inhibits proliferation by blocking at G2/M phase. May act as a negative regulator of IL1B transduction pathway in liver. Upon IL1B stimulus, cooperates with NLK to activate the transactivation activity of C/EBP subfamily members. Besides its function of transcription factor, acts as a cofactor of CEBPB to activate CEBPA and promote adipocyte differentiation. Regulates centrosome dynamics in a cell-cycle- and centriole-age-dependent manner. Forms 9-foci symmetrical ring scaffold around the mother centriole to control centrosome function and the interaction between centrioles and pericentriolar material.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
A synthetic peptide: Human ATF5
抗原种属
Human
基因ID
22809
Uniprot ID
Q9Y2D1
研究领域
Epigenetics and Nuclear Signaling
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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关键词

Transcription factor-like protein ODA-10Transcription factor ATFxNRIF3-associated proteinNap1NAP 1Cyclic AMP-dependent transcription factor ATF-5cAMP-dependent transcription factor ATF-5BZIP protein ATF7BZIP protein ATF 7AtfxAtf5Atf 5Activating transcription factor 5-α/βActivating transcription factor 5-α/betaActivating transcription factor 5-α/bActivating transcription factor 5-alpha/βActivating transcription factor 5-alpha/betaActivating transcription factor 5-alpha/bActivating transcription factor 5-a/βActivating transcription factor 5-a/betaActivating transcription factor 5-a/b

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