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Anti-ATF-5 Antibody (5U516)
还可以产品编号 TMAH-00086
Anti-ATF-5 Antibody (5U516) 是一种抗体，靶向 ATF-5。Anti-ATF-5 Antibody (5U516) 可用于 ELISA, WB, IHC。
Anti-ATF-5 Antibody (5U516)
Anti-ATF-5 Antibody (5U516)
还可以产品编号 TMAH-00086
Anti-ATF-5 Antibody (5U516) 是一种抗体，靶向 ATF-5。Anti-ATF-5 Antibody (5U516) 可用于 ELISA, WB, IHC。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-ATF-5 Antibody (5U516) is an antibody targeting ATF-5. Anti-ATF-5 Antibody (5U516) can be used in ELISA, WB, IHC. |
| Ig Type | Rabbit IgG |
| 克隆号 | 5U516 |
| 交叉反应 | Human, Rat |
| 验证活性 | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, THP-1 whole cell lysate, PC-3 whole cell lysate, Rat brain tissue -All lanes: ATF5 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 31 kDa -Observed band size: 35 kDa 2. IHC image of TMAH-00086 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| 应用 | ELISA, WB, IHC |
| 推荐剂量 | WB:1:500-1:5000; IHC:1:50-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. Important regulato... |
| 偶联 | Unconjugated |
| 免疫原 | A synthesized peptide derived from human ATF5 |
| 抗原种属 | Human |
| 基因ID | 22809 |
| Uniprot ID | |
| 研究领域 | Epigenetics and Nuclear Signaling |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
SCI 文献
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
关键词
Transcription factor-like protein ODA-10Transcription factor ATFxNRIF3-associated proteinNap1NAP 1Cyclic AMP-dependent transcription factor ATF-5cAMP-dependent transcription factor ATF-5BZIP protein ATF7BZIP protein ATF 7AtfxAtf5Atf 5Activating transcription factor 5-α/βActivating transcription factor 5-α/betaActivating transcription factor 5-α/bActivating transcription factor 5-alpha/βActivating transcription factor 5-alpha/betaActivating transcription factor 5-alpha/bActivating transcription factor 5-a/βActivating transcription factor 5-a/betaActivating transcription factor 5-a/b
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