Anti-Adenylosuccinate Lyase Antibody (9U559) is a Rabbit antibody targeting Adenylosuccinate Lyase. Anti-Adenylosuccinate Lyase Antibody (9U559) can be used in WB,ICC/IF,IP.

Rabbit IgG

9U559

Human

1. Anti-ADSL rabbit monoclonal antibody at 1:500 dilution.
-Lane A: HepG2 Whole Cell Lysate.
-Lane B: Jurkat Whole Cell Lysate.
-Lane C: Hela Whole Cell Lysate.
-Lane D: C6 Whole Cell Lysate.
-Lane E: Raw264.7 Whole Cell Lysate.
-Lane F: NIH-3T3 Whole Cell Lysate.
-Lane G: HCT-116 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:55 kDa.
-Observed band size:55 kDa.
2. Immunofluorescence staining of ADSL in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human ADSL monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).
3. ADSL was immunoprecipitated using:
-Lane A:0.5 mg HepG2 Whole Cell Lysate.
-2 µL anti-ADSL rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-ADSL rabbit monoclonal antibody, at 1:200 dilution.
-Secondary antibody:
-Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution.
-Developed using the DAB staining technique.
-Performed under reducing conditions.
-Predicted band size: 50 kDa.
-Observed band size: 50 kDa

WB: 1:500-1:2000; ICC-IF: 1:20-1:100; IP: 1-4 μL/mg of lysate

Monoclonal

Unconjugated

Recombinant Protein: Human ADSL / Adenylosuccinate Lyase protein (TMPY-02552)

Human