Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Wnt-C59 (C59) 是一种高效的、口服具有活力的PORCN 抑制剂(IC50:74 pM)。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 233 | 现货 | ||
2 mg | ¥ 329 | 现货 | ||
5 mg | ¥ 536 | 现货 | ||
10 mg | ¥ 828 | 现货 | ||
25 mg | ¥ 1,590 | 现货 | ||
50 mg | ¥ 2,480 | 现货 | ||
100 mg | ¥ 3,880 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 659 | 现货 |
产品描述 | Wnt-C59 (C59)(C59) is a highly effective and specific Wnt signaling antagonis with PORCN enzymatic activity. |
靶点活性 | Porcn:74 pM |
体外活性 | 在原位移植独立性MMTV-WNT1肿瘤的雌性裸鼠中,Wnt-C59(10 mg/kg)能够降低β-catenin靶向基因的表达,降低Wnt通路活性,抑制肿瘤细胞生长.14CREER/Rosa-SmoM2小鼠中,Wnt-C59(5 mg/kg)局部给药能够抑制细胞增殖. |
体内活性 | 在转染PORCN的无PORCN HT1080细胞中,Wnt-C59(100 nM )抑制PORCN活性。在转染WNT3A-V5的HeLa细胞中,Wnt-C59(10 -100 nM)能够抑制PORCN酰基转移酶活性。 |
激酶实验 | Aurora A radioactive Flashplate enzyme assay: Aurora A radioactive Flashplate enzyme assay is conducted to determine the nature and degree of MLN8237-mediated inhibition in vitro. Recombinant Aurora A is expressed in Sf9 cells and purified with GST affinity chromatography. The peptide substrate for Aurora A is conjugated with biotin (Biotin-GLRRASLG). Aurora A kinase (5 nM) is assayed in 50 mM Hepes (pH 7.5), 10 mM MgCl2, 5 mM DTT, 0.05% Tween 20, 2 μM peptide substrate, 3.3 μCi/mL [γ-33P]ATP at 2 μM, and increasing concentrations of MLN8237 by using Image FlashPlates. |
细胞实验 | Wnt-C59 (C59) is dissolved in DMSO (10 mM) and stored, and then diluted with appropriate medium before use[1]. Approximately, 1×104 cells are seeded in 24-well plates, and Wnt-C59 (5 μM, 10 μM, and 20 μM) is added the next day. Each group is tested in triplicate and control groups with addition of DMSO are also established. Cell confluence is determined by microscopy at 24, 48, 72, and 96 hours after seeding of cells. The IC50 of Wnt-C59 is determined by MTT assay, using 96-well dishes. Next day, various concentrations of Wnt-C59 are added, and cellular viabilities are measured by a spectrophotometer at both 24 and 48 hours. For sphere formation, approximately one hundred cells are seeded onto the Low Cell Bind Surface 24-well Nunc dish. Each group is done in triplicate and each well had 2 mL medium. Media are changed twice a week, and only half of the media is changed each time. Approximately, 1×103 cells are seeded for each well in the sphere inhibition assay. At 1 to 5 days after plating, all tested cells formed small spheres. Five days later, Wnt-C59 (1 μM, 5 μM, and 20 μM) is added into experimental groups. Abilities for cell growth and sphere images are compared and recorded at the end of the first, second, and third weeks after addition of Wnt-C59, or DMSO in control groups. The sphere growths are observed and recorded daily under microscopy, and the area of spheres is analyzed using Metamorph and recorded as average area (μm2)[1]. |
别名 | 4-(2-甲基-4-吡啶基)-N-[4-(3-吡啶基)苯基]苯乙酰胺, C59 |
分子量 | 379.45 |
分子式 | C25H21N3O |
CAS No. | 1243243-89-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 7.6 mg/mL (20 mM)
DMSO: 7.6 mg/mL (20 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
Ethanol / DMSO | 1 mM | 2.6354 mL | 13.177 mL | 26.3539 mL | 65.8848 mL |
5 mM | 0.5271 mL | 2.6354 mL | 5.2708 mL | 13.177 mL | |
10 mM | 0.2635 mL | 1.3177 mL | 2.6354 mL | 6.5885 mL | |
20 mM | 0.1318 mL | 0.6588 mL | 1.3177 mL | 3.2942 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Wnt-C59 1243243-89-1 Cytoskeletal Signaling Stem Cells Wnt/beta-catenin Porcupine Wnt WntC59 Inhibitor 4-(2-甲基-4-吡啶基)-N-[4-(3-吡啶基)苯基]苯乙酰胺 C59 Wnt-C-59 inhibit C 59 C-59 Wnt C59 inhibitor