Powder: -20°C for 3 years | In solvent: -80°C for 1 year
TB5 是一种选择性可逆 hMAO-B 有效抑制剂,Ki=0.11±0.01 μM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | ¥ 397 | 现货 | ||
10 mg | ¥ 666 | 现货 | ||
25 mg | ¥ 1,254 | 现货 | ||
50 mg | ¥ 1,995 | 现货 | ||
100 mg | ¥ 3,187 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 448 | 现货 |
产品描述 | TB5 is a potent, selective and reversible inhibitor of hMAO-B. |
靶点活性 | hMAO-B:0.11±0.01 μM. (Ki) |
体外活性 | TB5 and TB8 interacts with the catalytic site of hMAO-B and hMAO-A with a competitive mode of inhibition. TB5 exhibits the best inhibitory activity and higher selectivity toward hMAO-B, with Ki and SI values of 0.11±0.01 μM and 13.18, respectively. hMAO-B inhibition by compound TB5 and hMAO-A inhibition by compound TB8 are completely reversed after 24 h of dialysis. Cytotoxicity studies show TB5 is nontoxic at 5 and 25 μM with cell viabilities of 95.75% and 84.59 %, respectively[1]. |
体内活性 | Compounds are dissolved in DMSO (5 mg/mL) and diluted with PBS/EtOH (70:30). Kinetic analyses are carried out for TB5 and TB8 to understand the crucial interactions responsible for selectivity and potency. A set of Lineweaver–Burk plots are constructed in the absence and presence of various concentrations of compounds TB5 and TC8. The set consists of five graphs, each constructed by measuring MAO-B and MAO-A catalytic rates at different substrate concentrations (0.1-1 μM). The first Lineweaver–Burk plot is constructed in the absence of inhibitor, while the remaining four graphs are constructed in the presence of different concentrations of TB5 and TB8[1]. |
激酶实验 | Compounds are dissolved in DMSO (5 mg/mL) and diluted with PBS/EtOH (70:30).Kinetic analyses are carried out for TB5 and TB8. A set of Lineweaver–Burk plots are constructed in the absence and presence of various concentrations of compounds TB5 and TC8. The set consists of five graphs, each constructed by measuring MAO-B and MAO-A catalytic rates at different substrate concentrations (0.1-1 μM). The first Lineweaver–Burk plot is constructed in the absence of inhibitor, while the remaining four graphs are constructed in the presence of different concentrations of TB5 and TB8[1]. |
细胞实验 | In vitro cytotoxicity of brominated thiophene chalcones and standard MAO inhibitors are tested in human HepG2 hepatic cancer cells at three different concentrations (1, 5 and 25 μM)[1]. |
分子量 | 336.25 |
分子式 | C15H14BrNOS |
CAS No. | 948841-07-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 55 mg/mL (163.57 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.974 mL | 14.8699 mL | 29.7398 mL | 74.3494 mL |
5 mM | 0.5948 mL | 2.974 mL | 5.948 mL | 14.8699 mL | |
10 mM | 0.2974 mL | 1.487 mL | 2.974 mL | 7.4349 mL | |
20 mM | 0.1487 mL | 0.7435 mL | 1.487 mL | 3.7175 mL | |
50 mM | 0.0595 mL | 0.2974 mL | 0.5948 mL | 1.487 mL | |
100 mM | 0.0297 mL | 0.1487 mL | 0.2974 mL | 0.7435 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
TB5 948841-07-4 Metabolism Neuroscience Monoamine Oxidase MAO TB 5 Inhibitor inhibit TB-5 inhibitor