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SEA0400

SEA0400

产品编号 T3508   CAS 223104-29-8
别名: SEA 0400, 2-[4-[(2,5-二氟苯基)甲氧基]苯氧基]-5-乙氧基苯胺, SEA-0400

SEA0400 是 Na+/Ca2+ 交换剂的选择性抑制剂,在培养的神经元、星形胶质细胞和小胶质细胞中,能够抑制 Na+-依赖性 Ca2+的吸收,IC50值为 5-33 nM。

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SEA0400 Chemical Structure
SEA0400, CAS 223104-29-8
规格 价格/CNY 货期 数量
1 mg ¥ 471 现货
2 mg ¥ 686 现货
5 mg ¥ 1,160 现货
10 mg ¥ 1,860 现货
25 mg ¥ 3,430 现货
50 mg ¥ 4,990 现货
100 mg ¥ 6,960 现货
500 mg ¥ 14,200 现货
1 mL * 10 mM (in DMSO) ¥ 1,280 现货
产品目录号及名称: SEA0400 (T3508)
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生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 SEA0400 is a selective inhibitor of the Na+/Ca2+ exchanger.
靶点活性 NCX:5 -33 nM.
体外活性 SEA0400 inhibits Na+-dependent 45Ca2+ uptake in cultured neurons, astrocytes, and microglia. IC50 values of SEA0400 are 33 nM (neurons), 5.0 nM (astrocytes), and 8.3 nM (microglia)[1]. SEA0400 prevents sodium nitroprusside (SNP) to increase ERK and p38 MAPK phosphorylation, and production of reactive oxygen species (ROS) in an extracellular Ca2+-dependent manner[2].
体内活性 SEA0400 (3 mg/kg + 3 mg/kg/h for 2 h, i.v.) attenuates the infarct volume in the cerebral cortex and striatum without affecting the mean regional cortical blood flow in anesthetized rats[1]. SEA0400 protects against the dopaminergic neurotoxicity (determined by dopamine levels in the midbrain and striatum, tyrosine hydroxylase immunoreactivity in the substantia nigra and striatum, striatal dopamine release, and motor deficits) in MPTP-treated C57BL/6J mice[3].
激酶实验 Na+-Ca2+ exchange activity is determined by assaying Na+-dependent 45Ca2+ uptake as reported previously. Briefly, the cells are preincubated in Hanks' balanced saline solution (HBSS) for 20 min, and the medium is switched to HBSS containing 45Ca2+?and incubated for 5 min. To increase intracellular Na+?concentration, 1 mM ouabain plus 20 μM monensin (for astrocytes and microglia) and 10 μM monensin (for neurons) are used. Monensin is added simultaneously with the isotope. Ouabain is added 5 min before monensin in astrocytes and microglia. SEA0400 and KB-R7943 are added 5 min before monensin and present during 45Ca2+?uptake reaction.
细胞实验 SEA0400 is dissolved in DMSO (final concentration 0.1%). Cells, plated in 96-well plastic tissue culture plates, are incubated at 37°C for 30 min in normal or Ca2+-free HBSS containing 10 μM H2DCF-DA and 0.25 μg/mL Cremophor EL, and then rinsed twice with normal HBSS to remove excess dye. The cells are reperfused in normal HBSS for 1 h, and the conversion of H2DCF-DA to its fluorescent product dichlorofluorescein by ROS, presumably Water2 and hydroxyl radical, is determined with excitation at 485 nm and emission at 535 nm using a Wallac Multilabel counter. ROS production is expressed as a percentage of control cells. The linearity and sensitivity of ROS assay are confirmed using Water2 prior to the experiment. SEA0400 at the indicated concentrations is added 10 min before Ca2+?reperfusion and present until assay.
别名 SEA 0400, 2-[4-[(2,5-二氟苯基)甲氧基]苯氧基]-5-乙氧基苯胺, SEA-0400
分子量 371.38
分子式 C21H19F2NO3
CAS No. 223104-29-8

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 50 mg/mL (134.63 mM)

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6927 mL 13.4633 mL 26.9266 mL 67.3165 mL
5 mM 0.5385 mL 2.6927 mL 5.3853 mL 13.4633 mL
10 mM 0.2693 mL 1.3463 mL 2.6927 mL 6.7316 mL
20 mM 0.1346 mL 0.6732 mL 1.3463 mL 3.3658 mL
50 mM 0.0539 mL 0.2693 mL 0.5385 mL 1.3463 mL
100 mM 0.0269 mL 0.1346 mL 0.2693 mL 0.6732 mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
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输入分子式,点击计算,可计算出产品的分子量。

参考文献

1. Matsuda T, et al. SEA0400, a novel and selective inhibitor of the Na+-Ca2+ exchanger, attenuates reperfusion injury in the in vitro and in vivo cerebral ischemic models. J Pharmacol Exp Ther. 2001 Jul;298(1):249-56. 2. Nashida T, et al. The specific Na(+)/Ca(2+) exchange inhibitor SEA0400 prevents nitric oxide-induced cytotoxicity in SH-SY5Y cells. Neurochem Int. 2011 Aug;59(1):51-8. 3. Ago Y, et al. SEA0400, a specific Na+/Ca2+ exchange inhibitor, prevents dopaminergic neurotoxicity in an MPTP mouse model of Parkinson's disease. Neuropharmacology. 2011 Dec;61(8):1441-51.
Benzamil CGP37157 KB-R7943 mesylate SN 6 Terfenadine PPADS tetrasodium FR183998 free base SM-6586

相关化合物库

该产品包含在如下化合物库中:
已知活性化合物库 神经信号分子库 临床前化合物库 钠通道分子库 抗高血压化合物库 离子通道库 钙通道分子库 抗癌化合物库 干细胞分化化合物库 抑制剂库

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
每只动物体重
g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
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% Tween 80
% ddH2O
计算 重置

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

SEA0400 223104-29-8 Membrane transporter/Ion channel Metabolism Na+/Ca2+ Exchanger Calcium Channel SEA 0400 2-[4-[(2,5-二氟苯基)甲氧基]苯氧基]-5-乙氧基苯胺 inhibit SEA-0400 Inhibitor inhibitor

 

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