Powder: -20°C for 3 years | In solvent: -80°C for 1 year
XI-006 (XI-006) 是一种DNA 破坏剂、抗肿瘤剂和p53的激活剂。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 427 | 现货 | ||
2 mg | ¥ 616 | 现货 | ||
5 mg | ¥ 997 | 现货 | ||
10 mg | ¥ 1,660 | 现货 | ||
25 mg | ¥ 3,660 | 现货 | ||
50 mg | ¥ 5,290 | 现货 | ||
100 mg | ¥ 7,490 | 现货 | ||
500 mg | ¥ 14,900 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 942 | 现货 |
产品描述 | XI-006 (XI-006) suppresses MDMX with IC50 of 2.5 μM, leading to enhanced p53 stabilization/activation and DNA damage, and also regulates MDM2, an E3 ligase. |
靶点活性 | MDMX:2.5 μM |
体外活性 | XI-006 decreases both the MDMX mRNA and protein in MCF-7 cells. XI-006 induces expression of p53 as well as well-characterized p53-target gene, p21 and MDM2, in a dose-dependent manner in MCF-7 cells. XI-006 extends the half-life of p53 from 20 to 30 minutes to more than 3 hours as revealed by cycloheximide chase assays in MCF-7 cells. XI-006 also activates p53 and induces p21 and MDM2 expression in LNCaP prostate and A549 lung cancer cells. XI-006 increases the mRNA levels of proapoptotic genes including PUMA, BAX, and PIG3 in a dose-dependent manner in MCF-7 cells. XI-006 results in a significant increase in the numbers of sub-G0/G1 cells as well as G2 arrest. XI-006 also results in more than 40% of cells dying via apoptosis and decreases cell viability in A549 and LNCaP cells. [1] XI-006 inhibits biosynthesis of nucleic acids and proteins in L1210 cells. [2] XI-006 interacts with DNA repair to activate the DNA damage repair pathway in three species (S. cerevisiae, S. pombe and H. sapiens). [3] XI-006 acts as cytotoxic agent in the G/R-luc astrocytoma cell line with GI50 of 117 nM. [4] |
细胞实验 | MCF-7 cells treated with dimethyl sulfoxide (DMSO), nutlin-3a, or NSC-207895 are permeabilized with cold 70% ethanol overnight, and stained with a solution containing 50 μg/mL propidium iodide and 20 μg/mL RNase A at 37°C for 20 minutes. The cells are then subjected to flow cytometry analysis. The FlowJo software is used to calculate percentages of cells in each cell cycle phase. For terminal deoxynucleotidyl transferase–mediated dUTP nick end labeling (TUNEL) staining, MCF-7 cells treated with the NSC-207895 for 2 days are fixed with 4% paraformadelhyde for 1 hour, and then subjected to dUTP labeling using In Situ Cell Death Detection Kit TMR Red according to the manufacturer's protocol. For quantitation, at least 300 cells are randomly chosen and the numbers of TUNEL-positive cells are counted. (Only for Reference) |
别名 | NSC 207895, 可可碱 |
分子量 | 279.25 |
分子式 | C11H13N5O4 |
CAS No. | 58131-57-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 55 mg/ml (196.96 mM)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
H2O: < 1 mg/mL (insoluble or slightly soluble)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.581 mL | 17.9051 mL | 35.8102 mL | 89.5255 mL |
5 mM | 0.7162 mL | 3.581 mL | 7.162 mL | 17.9051 mL | |
10 mM | 0.3581 mL | 1.7905 mL | 3.581 mL | 8.9526 mL | |
20 mM | 0.1791 mL | 0.8953 mL | 1.7905 mL | 4.4763 mL | |
50 mM | 0.0716 mL | 0.3581 mL | 0.7162 mL | 1.7905 mL | |
100 mM | 0.0358 mL | 0.1791 mL | 0.3581 mL | 0.8953 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
XI-006 58131-57-0 Apoptosis Mdm2 p53 LPS MDMX XI006 Inhibitor NSC-207895 inhibit MDM2 XI 006 MDM-2/p53 NSC 207895 NSC207895 可可碱 inhibitor