Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Hydroxyfasudil Hydrochloride (HA 1100 hydrochloride) 是一种ROCK 抑制剂,对ROCK1和ROCK2的IC50值分别为 0.73 和 0.72 μM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 228 | 现货 | ||
2 mg | ¥ 329 | 现货 | ||
5 mg | ¥ 578 | 现货 | ||
10 mg | ¥ 872 | 现货 | ||
25 mg | ¥ 1,770 | 现货 | ||
50 mg | ¥ 3,350 | 现货 | ||
100 mg | ¥ 4,870 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 638 | 现货 |
产品描述 | Hydroxyfasudil Hydrochloride (HA 1100 hydrochloride) is a ROCK1/2 inhibitor (IC50s: 0.73/0.72 μM). |
靶点活性 | ROCK1:0.73 μM, ROCK2:0.72 μM |
体外活性 | Hydroxyfasudil has a weak inhibition of PKA (IC50: 37 μM), 50-fold higher than those of the ROCKs. Hydroxyfasudil increases eNOS mRNA levels (EC50: 0.8 ± 0.3 μM). Hydroxyfasudil (0-100 μM) concentration-dependently increases eNOS activity and stimulates NO production in HAEC. Hydroxyfasudil (10 μM) increases the half-life of eNOS mRNA from 13 to 16 hours but does not affect eNOS promoter activity (0.1-100 μM). |
体内活性 | Hydroxyfasudil (10 mg/kg, i.p.) markedly increases both the maximal and average voided volumes in SD rats. Hydroxyfasudil also markedly reduces the maximal detrusor pressure. In spontaneously hypertensive rats (SHRs), Hydroxyfasudil (3 mg/kg, i.p.) inhibits hypercontractility induced by norepinephrine. Additionally, Hydroxyfasudil (3/10 mg/kg, i.p.) significantly ameliorates decreased penile cGMP contents in rats. |
动物实验 | Hydroxyfasudil is formulated in saline.Micturition behavior is studied after administration of either Hydroxyfasudil (10 mg/kg, i.p.) or a corresponding volume of saline. Each rat is placed in a metabolic cage containing a urine collection funnel that is placed over an electronic balance. The balance is connected to a personal computer via a multiport controller and used to measure the cumulative weight of the collected urine. Every 150 s during a continuous 24-h period, the computer samples and records the data for the micturition frequency and volumes. The micturition reflex parameters that are collected include: urine volume per micturition, maximal micturition volume, micturition frequency, and total urine output in the Hydroxyfasudil- or vehicle-treated animals. Each monitoring session started at 18.00 hours. Prior to being placed in the metabolic cage at the start of each experimental period, the animals received either a single injection of Hydroxyfasudil (10 mg/kg) dissolved in saline or an injection of saline without the inhibitor. |
别名 | Hydroxyfasudil (HA-1100) HCl, 羟基法舒地尔盐酸盐, RHO-激酶抑制剂, HA 1100 hydrochloride |
分子量 | 343.83 |
分子式 | C14H18ClN3O3S |
CAS No. | 155558-32-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 62 mg/mL(180.3 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.9084 mL | 14.5421 mL | 29.0841 mL | 72.7104 mL |
5 mM | 0.5817 mL | 2.9084 mL | 5.8168 mL | 14.5421 mL | |
10 mM | 0.2908 mL | 1.4542 mL | 2.9084 mL | 7.271 mL | |
20 mM | 0.1454 mL | 0.7271 mL | 1.4542 mL | 3.6355 mL | |
50 mM | 0.0582 mL | 0.2908 mL | 0.5817 mL | 1.4542 mL | |
100 mM | 0.0291 mL | 0.1454 mL | 0.2908 mL | 0.7271 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Hydroxyfasudil Hydrochloride 155558-32-0 Cell Cycle/Checkpoint Cytoskeletal Signaling Stem Cells ROCK Hydroxyfasudil (HA-1100) HCl HA-1100 Hydroxyfasudil Rho-associated kinase HA 1100 羟基法舒地尔盐酸盐 inhibit HA1100 HA1100 Hydrochloride Rho-kinase RHO-激酶抑制剂 Rho-associated protein kinase HA-1100 Hydrochloride ROK HA 1100 hydrochloride Inhibitor HA 1100 Hydrochloride inhibitor