Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Dynasore (Dynamin Inhibitor I) 是一种可渗透细胞的化学物质,可逆且非竞争性地抑制 dynamin 1/2 的 GTPase 活性。 它还抑制线粒体动力蛋白 Drp1,对其他小 GTPase 没有影响。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 163 | 现货 | ||
5 mg | ¥ 359 | 现货 | ||
10 mg | ¥ 578 | 现货 | ||
25 mg | ¥ 1,070 | 现货 | ||
50 mg | ¥ 1,860 | 现货 | ||
100 mg | ¥ 2,630 | 现货 | ||
200 mg | ¥ 3,960 | 现货 | ||
500 mg | ¥ 6,360 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 638 | 现货 |
产品描述 | Dynasore (Dynamin Inhibitor I), a cell-permeable chemical, can reversibly and non-competitivity inhibit GTPase activity of dynamin 1/2 (IC50: 15 μM in a cell-free assay). It also inhibits the mitochondrial dynamin Drp1, with no effect against other small GTPase. |
靶点活性 | Dynamin1/2:15 μM |
体内活性 | Dynasore ameliorates the motor dysfunction greatly at 3, 7, and 10 days after SCI in rats. Dynasore markedly enhances motor function which may be through inhibiting the activation of neuronal mitochondrial apoptotic pathway and astrocytic proliferation in rats after SCI[4]. |
激酶实验 | RIP1 kinase assay: Phosphorylation of RIP1 requires its kinase activity. Expression constructs of FLAGtagged wild-type (WT) or a kinase-inactive point mutant of RIP1 (K45M) are are transfected into 293T cells and RIP1 kinase assay is performed as described in the Methods in the presence of [γ-32P]ATP for 30 min at 30℃. Samples are subjected to SDS-PAGE and RIP1 band is visualized by autoradiography. Relative intensities of radioactive bands are quantified and are shown (ratio) in this and all other autoradiographs. In parallel to kinase reactions, a sample of beads is subjected to western blot analysis using anti-RIP1 antibody to ensure equal protein amounts in kinase reactions. |
细胞实验 | Mouse ventricular myocytes are isolated from male adult C6/Black mouse. Cardiomyocytes subjects to 2 hours of drug treatment followed by oxidative stress (30 μM Water2 for 35 min). For ATP supplement experiments, the cells are treated with 3 mM ATP for 30 min before exposure to Water2. Cardiomyocyte survival and viability are analyzed by trypan blue exclusion (TBE) assay[3]. |
别名 | Dynamin Inhibitor I |
分子量 | 322.31 |
分子式 | C18H14N2O4 |
CAS No. | 304448-55-3 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 1.6 mg/mL (5 mM)
DMSO: 32.2 mg/mL (100 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
Ethanol / DMSO | 1 mM | 3.1026 mL | 15.513 mL | 31.026 mL | 77.5651 mL |
5 mM | 0.6205 mL | 3.1026 mL | 6.2052 mL | 15.513 mL | |
DMSO | 10 mM | 0.3103 mL | 1.5513 mL | 3.1026 mL | 7.7565 mL |
20 mM | 0.1551 mL | 0.7757 mL | 1.5513 mL | 3.8783 mL | |
50 mM | 0.0621 mL | 0.3103 mL | 0.6205 mL | 1.5513 mL | |
100 mM | 0.031 mL | 0.1551 mL | 0.3103 mL | 0.7757 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
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