Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Alda-1是一种 ALDH2 激动剂,是野生型 ALDH2*1 和亚洲 E487K 突变体 ALDH2*2 形式的线粒体醛脱氢酶 2 的细胞渗透激活剂。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 158 | 现货 | ||
5 mg | ¥ 333 | 现货 | ||
10 mg | ¥ 538 | 现货 | ||
25 mg | ¥ 697 | 现货 | ||
50 mg | ¥ 862 | 现货 | ||
100 mg | ¥ 1,530 | 现货 | ||
200 mg | ¥ 2,290 | 现货 | ||
500 mg | ¥ 3,890 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 597 | 现货 |
产品描述 | Alda-1 is an ALDH2 agonist, a cell-permeable activator of both the wild-type ALDH2*1 and the Asian E487K mutant ALDH2*2 forms of mitochondrial aldehyde dehydrogenase 2 (mtALDH2). |
激酶实验 | Lipid kinase activity : IC50 values are measured using a standard lipid kinase activity with PI as a substrate. (i)100 μM cold ATP is used instead of 10 μM, (ii) the DMSO concentration is 1%, and (iii) [γ-33P]ATP is used instead of [γ-32P]ATP. The TLC plates are quantified using a phosphorimager screen. The reported IC50 values are determined by non-linear regression analysis on the basis of at least three independent experiments repeated across multiple preparations of recombinant protein. |
细胞实验 | Spleen cells (4×106 cells/mL) are stimulated by optimal concentrations of concanavalin A (Con A; 2.5 μg/mL and 0.6 μg/mL) and lipopolysaccharide (LPS, 5 μg/mL) and are incubated in 96-well plates at final volume of 0.2 mL for 72 h. Cell proliferation is determined by adding 0.5 μCi of [3H]-thymidine per well at 16 h before the end of the incubation. The cultures are harvested with an automatic cell harvester, and [3H] thymidine incorporation is assessed using a liquid scintillation counter. |
别名 | Alda 1 |
分子量 | 324.16 |
分子式 | C15H11Cl2NO3 |
CAS No. | 349438-38-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 32.4 mg/mL(100 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.0849 mL | 15.4245 mL | 30.849 mL | 77.1224 mL |
5 mM | 0.617 mL | 3.0849 mL | 6.1698 mL | 15.4245 mL | |
10 mM | 0.3085 mL | 1.5424 mL | 3.0849 mL | 7.7122 mL | |
20 mM | 0.1542 mL | 0.7712 mL | 1.5424 mL | 3.8561 mL | |
50 mM | 0.0617 mL | 0.3085 mL | 0.617 mL | 1.5424 mL | |
100 mM | 0.0308 mL | 0.1542 mL | 0.3085 mL | 0.7712 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Alda-1 349438-38-6 Apoptosis Metabolism Dehydrogenase inhibit Aldehyde Dehydrogenase (ALDH) Inhibitor Alda1 Alda 1 inhibitor